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A noninvasive method of assessing the motion of the subtalar joint was developed for use in clinical and research settings. Anatomical reference frames for the calcaneus and talus were produced using a marker placement model utilizing 14 markers. An asymptomatic individual was tested during barefoot walking with a CODA MPX30 system. Intertrial variability and motion patterns, in all three planes, of the calcaneus with respect to the talus were analyzed as part of a validation study. The observed patterns in all three planes were found to have good face validity with published literature as well as good consistency during stance. The findings of this study support the further use of this model in both clinical and research settings, allowing investigation of the motion patterns of a larger cohort than has hitherto been possible.