Eldor R, Raz I, Ben YA, et al: New and experimental approaches to treatment of diabetic foot ulcers: a comprehensive review of emerging treatment strategies. .Diabet Med 21::1161. ,2004. .
Phillips TJ: Successful methods of treating leg ulcers: the tried and true, plus the novel and new. .Postgrad Med 105::159. ,1999. .
Aminian B, Shams M, Soveyd M, et al: Topical autologous platelet-derived growth factors in the treatment of chronic diabetic ulcers. .Arch Iran Med (April):3. ,2000. .
Margolis DJ, Kantor J, Santanna J, et al: Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. .Diabetes Care 24::483. ,2001. .
Trent JT, Falabella A, Eaglstein WH, et al: Venous ulcers: pathophysiology and treatment options. .Ostomy Wound Manage 51::38. ,2005. .
Abbade LP, Lastoria S, de Almeida Rollo H, et al: A sociodemographic, clinical study of patients with venous ulcer. .Int J Dermatol 44::989. ,2005. .
Agren MS, Eaglstein WH, Ferguson MW, et al: Causes and effects of the chronic inflammation in venous leg ulcers. .Acta Derm Venereol Suppl (Stockh) 210::3. ,2000. .
Raffetto JD, Vasquez R, Goodwin DG, et al: Mitogen-activated protein kinase pathway regulates cell proliferation in venous ulcer fibroblasts. .Vasc Endovasc Surg 40::59. ,2006. .
Sieggreen MY, Kline RA: Arterial insufficiency and ulceration: diagnosis and treatment options. .Nurse Pract 29::46. ,2004. .
Weitz JI, Byrne J, Clagett GP, et al: Diagnosis and treatment of chronic arterial insufficiency of the lower extremities: a critical review. .Circulation 94::3026. ,1996. .
Bouza C, Munoz A, Amate JM: Efficacy of modern dressings in the treatment of leg ulcers: a systematic review. .Wound Repair Regen 13::218. ,2005. .
Sharma S, McAleer JP: Debriding wounds with the Versajet hydrosurgery system. .Podiatry Manage 24::127. ,2005. .
Fu X, Li X, Cheng B, et al: Engineered growth factors and cutaneous wound healing: success and possible questions in the past 10 years. .Wound Repair Regen 13::122. ,2005. .
Ross R, Raines EW, Bowen-Pope DF: The biology of platelet-derived growth factor. .Cell 46::155. ,1986. .
Mulder GD: Standardizing wound treatment procedures for advanced technologies. .JAPMA 92::7. ,2002. .
Eppley BL, Woodell JE, Higgins J: Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. .Plast Reconstr Surg 114::1502. ,2004. .
Crovetti G, Martinelli G, Issi M, et al: Platelet gel for healing cutaneous chronic wounds. .Transfus Apher Sci 30::145. ,2004. .
The efficacy of concentrated autologous platelet-derived growth factors in the healing and closure of chronic lower-extremity wounds was evaluated in 24 patients with 33 lower-extremity wounds treated previously for at least 6 months using traditional methods. Surgical wound debridement was performed to convert chronic ulcers into acute wounds. Concentrated autologous platelet-derived growth factors and thrombin were applied to the wound bases and protected with a nonadhering compression dressing that remained intact for 7 days. Wounds were evaluated and the concentrate was reapplied every 2 weeks. Wound closure and complete epithelialization was achieved in 20 wounds. Seventy-five percent or greater wound closure was obtained in three wounds, 50% to 74% closure in three wounds, and 25% to 49% closure in two wounds. Five wounds displayed no improvement. Mean time to complete closure was 11.15 weeks. The application of concentrated autologous platelet-derived growth factors and thrombin resulted in substantial wound healing and wound-diameter reduction. This technique constitutes a safe and effective treatment option and avoids lengthy treatment periods that increase the potential for infection. (J Am Podiatr Med Assoc 96(6): 482–488, 2006)